Postdoctoral Researcher in Protein Engineering
KU Leuven
Enzymatic DNA synthesis could deliver longer, purer and greener oligonucleotides than phosphoramidite chemistry. It requires an enzyme that is template-independent, adds exactly one nucleotide per cycle, accepts 3′-reversible terminators and withstands process conditions. This translational, KU Leuven-funded project continues the development of a recently discovered engineered hyperthermophilic polymerase with template-independent activity. You will improve it through directed evolution and structure-guided design. Your targets are terminator acceptance, coupling efficiency and control over st
View position